Isothermal DNA and RNA amplification
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Low Temperature DNA and RNA copying
Low Temperature DNA and RNA copying
Polymerase chain reaction (PCR) PCR was developed by Kary Mullis in 1983, which he was awarded the Nobel Prize in Chemistry in 1993 is a method widely used for DNA and RNA amplification which relies heavily on thermal cycling. Even though methods without thermal cycling exist, often they are not sensitive enough .
A new study published in the journal Angewandte Chemie introduced a new reliable method for DNA and RNA copying in low temperatures that can be easily be used outside of the lab, in the field. Named as Cas9nAR, in short for Cas9n nickase-based amplification reaction, can be used to amplify a target fragment from genomic DNA at a constant temperature of 37oC.
The method exhibits a zeptomolar limit of detection (2 copies in 20 μL of reaction system) within 60 min and a single‐base discrimination capability. Accorting to the researchers at the East China University of Science & Technology, Shanghai, China, :
Cas9nAR holds great potential to become a routine assay for the quantitative detection of nucleic acids in basic and applied studies.
Journal Reference:
- Ting Wang, Yong Liu, Huan-Huan Sun, Bin-Cheng Yin, Bang-Ce Ye. An RNA-Guided Cas9 Nickase-Based Method for Universal Isothermal DNA Amplification. Angewandte Chemie International Edition, 2019; DOI: 10.1002/anie.201901292
